Spider Queen may refer to:
- Corpse Spiders skill with the rune of the same name.
- Queen Araneae, the broodmother of spiders and a boss in Diablo III.
- The Spider Queen’s Grasp, a legendary ceremonial knife.
- Spirit of Arachyr Set may summon a Spider Queen to Witch Doctor's side.
- Spider Queens are often referenced in fantasy (and sometimes science fiction), although in real life, spiders do not live in colonies and do not have a designated queen (that is, broodmother).